!!How to grow a tooth May 5, 2004 Kings College London New advances in stem cell biology and tissue engineering are leading to the development of cutting edge approaches to dentistry both in the repair and replacement of teeth. The term ‘Regenerative Dentistry’ has been used by Professor Paul Sharpe of the Dental Institute, King’s College London to describe his improvements in the field that look set to revolutionise dentistry. He tells the AAAS Annual Meeting in Seattle* that by understa ...

A dual role for Ikk alpha in tooth development. February 2004 By Ohazama A, Hu Y, Schmidt-Ullrich R, Cao Y, Scheidereit C, Karin M, Sharpe PT. Developmental Cell You can view the abstract online. A subscription is required to view the full text or it can be purchased online. KK alpha is a component of the I kappa B kinase (IKK) complex that plays a key role in the activation of NF-kappa B. In Ikk alpha mutant mice and mice expressing a transdominant negative mutant of I kappa B alpha (cI k ...

Altered gene expression in human cleidocranial dysplasia dental pulp cells. January 2005 By Chen S, Santos L, Wu Y, Vuong R, Gay I, Schulze J, Chuang HH, Macdougall M. Archives of Oral Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Cleidocranial dysplasia (CCD) is an autosomal dominant disorder characterised by defects of bone and tooth development. The dental manifestations in CCD patients include supernu ...

Analysis of the odontogenic and osteogenic potentials of dental pulp in vivo using a Col1a1-2.3-GFP transgene 2003 By ALEN BRAUT, EDWARD J. KOLLAR and MINA MINA The International Journal of Developmental Biology Please visit the web site to view the article in its entirety. ABSTRACT Recently, transgenic mice that carry a Green Fluorescent Protein (GFP) reporter gene fused to 2.3 kb fragment of rat Col1a1 regulatory sequences (pOBCol2.3GFPemd) were generated. In the present study, we have ...

Are Cementoblasts a Subpopulation of Osteoblasts or a Unique Phenotype? 2005 D.D. Bosshardt Journal of Dental Research 84(5):390-406, 2005 You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Experimental studies have shown a great potential for periodontal regeneration. The limitations of periodontal regeneration largely depend on the regenerative potential at the root surface. Cellular intrinsic fiber cementum (CIFC), so-calle ...

Baby teeth provide new stem cell source April 23, 2003 By Niall Guerin Media52 Researchers have found a new source for stem cells – baby teeth. The announcement was made earlier this month when the scientists published their findings in the Proceedings of the National Academy of Sciences. Children develop about 20 deciduous or “baby teeth” between the ages of 1 and 6. After the age of six, those teeth are replaced with a new adult set. The scientists found that the dental pulp of the baby ...

Baby teeth revealed as source of stem cells April 21, 2003 By Catherine Zandonella NewScientist.com The tooth fairy could soon face competition for baby teeth from scientists who have discovered the teeth are a source of stem cells. The cells could help repair damaged teeth and perhaps even treat neural injuries or degenerative diseases. Currently, researchers can isolate two types of stem cells. Embryonic stem cells can develop into any cell in the body, but their harvesting requires the ...

Behavior of human periodontal ligament cells on CO2 laser irradiated dentinal root surfaces: an in vitro study. December 2004 By Pant V, Dixit J, Agrawal AK, Seth PK, Pant AB. Journal of Periodontal Research You can view the abstract online. A subscription is required to view the full text. OBJECTIVE: The aim of this study was to investigate the in vitro attachment behavior of human periodontal ligament fibroblasts on periodontally involved root surface after conditioning with CO2 laser an ...

Bioengineered Teeth from Cultured Rat Tooth Bud Cells 2004 By M.T. Duailibi4, S.E. Duailibi4, C.S. Young2, J.D. Bartlett2, J.P. Vacanti3, and P.C. Yelick2,* 1 University Federal of São Paulo, Department of Otorhinolaryngology and Human Communication Disorders, São Paolo, Brazil; 2 Department of Cytokine Biology, The Forsyth Institute, 140 The Fenway, and Department of Oral and Developmental Biology, Harvard Medical School, Boston, MA 02115, USA; and 3 Department of Surgery, Massachusetts ...

Comparison of Stem-cell-mediated Osteogenesis and Dentinogenesis 2003 By S. Batouli 1, M. Miura 1, J. Brahim 2, T.W. Tsutsui 1, L.W. Fisher 1, S. Gronthos 3, P. Gehron Robey 1, and S. Shi 1 1 Craniofacial and Skeletal Diseases Branch, 2 Clinical Research Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Department of Health and Human Services, Building 30, Room 222, NIDCR/NIH, 30 Convent Drive MSC-4320, Bethesda, MD 20892; 3 Mesenchymal Stem Cel ...

Continuous tooth replacement: the possible involvement of epithelial stem cells. June 2004 By Huysseune A, Thesleff I. Bioessays You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Epithelial stem cells have been identified in integumental structures such as hairs and continuously growing teeth of various rodents, and in the gut. Here we propose the involvement of epithelial stem cells in the continuous tooth replaceme ...

Cranial neural crest-derived mesenchymal proliferation is regulated by Msx1-mediated p19(INK4d) expression during odontogenesis. September 2003 By Han J, Ito Y, Yeo JY, Sucov HM, Maas R, Chai Y. Developmental Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Neural crest cells are multipotential progenitors that contribute to various cell and tissue types during embryogenesis. Here, we have investigated the m ...

Cultured human and rat tooth papilla cells induce hair follicle regeneration and fiber growth. December 2004 By Reynolds AJ, Jahoda CA. Differentiation You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The mesenchymal-epithelial interactions that characterize the early stages of tooth and hair follicle morphogenesis share certain similarities, and there is increasing evidence that mesenchymal cells derived from both ...

Cytotoxicity of two concentrations of a dentine bonding agent on mouse 3T3 and human pulp fibroblast cell-lines. October 2004 By Grobler SR, Olivier A, Moodley D, van W Kotze TW. SADJ Abstract The objective of this study was to investigate the cytotoxicity and effect of concentration of a recent dentine bonding agent on a mouse fibroblast cell-line (3T3) and four different human pulp fibroblast cell-lines. A mouse fibroblast 3T3 cell-line and 4 different human pulp fibroblast cell lines wer ...

Dental neuroplasticity, neuro-pulpal interactions, and nerve regeneration February 2003 By Margaret R. Byers, Hironobu Suzuki , & Takeyasu Maeda Microscopy Research and Technique You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract This review covers current information about the ability of dental nerves to regenerate and the role of tooth pulp in recruitment of regenerating nerve fibers. In addition, the participation ...

Dental Pulp Cells May Hold Key To Treatment Of Parkinson's Disease May 5, 2004 ScienceDaily ANN ARBOR, Mich. -- Cells derived from the inside of a tooth might someday prove an effective way to treat the brains of people suffering from Parkinson's disease. A study in the May 1 issue of the European Journal of Neuroscience shows dental pulp cells provide great support for nerve cells lost in Parkinson's disease and could be transplanted directly into the affected parts of the brain. The study' ...

Dental pulp fibroblasts contain target cells for lysophosphatidic Acid. June 2004 By Gruber R, Kandler B, Jindra C, Watzak G, Watzek G. J Dent Res You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Lysophosphatidic acid (LPA) is a locally produced bioactive phospholipid which is involved in tissue repair. The objective of this study was to determine whether dental pulp tissue also responds to the phospholipid. Effects ...

Dentin Regeneration by Dental Pulp Stem Cell Therapy with Recombinant Human Bone Morphogenetic Protein 2 2004 By . Iohara 1,2, M. Nakashima1,*, M. Ito,3, M. Ishikawa ,1, A. Nakasima ,2, and A. Akamine 1 1 Department of Clinical Oral Molecular Biology, Division of Oral Rehabilitation, 2 Department of Orthodontics, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812-8582, Japan; and 3 Department of First Ana ...

Dentonin, a Fragment of MEPE, Enhanced Dental Pulp Stem Cell Proliferation 2004 By H. Liu 1,2, W. Li1, C. Gao 1, Y. Kumagai 3, R.W. Blacher 3, and P.K. DenBesten 1,* 1 Box 0640, University of California, San Francisco, CA 94143-0640, USA; 2 Peking University School of Stomatology, Beijing, China; and 3 Acologix, Emeryville, CA, USA; Journal of Dental Research You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Matrix ext ...

Development of teeth in chick embryos after mouse neural crest transplantations May 2003 By Mitsiadis TA, Cheraud Y, Sharpe P, Fontaine-Perus J. DEVELOPMENTAL BIOLOGY Please visit the web site to view the article in its entirety. Abstract Teeth were lost in birds 70–80 million years ago. Current thinking holds that it is the avian cranial neural crest-derived mesenchyme that has lost odontogenic capacity, whereas the oral epithelium retains the signaling properties required to induce odon ...

Developmental analysis and computer modelling of bioengineered teeth. February 2005 By Young CS, Kim SW, Qin C, Baba O, Butler WT, Taylor RR, Bartlett JD, Vacanti JP, Yelick PC. Archives of Oral Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Here we present the developmental progression of bioengineered pig teeth from 1 to 25 weeks of development. We demonstrate that 2-25 week implants contained embryonic ...

Developmental expression of Smad1-7 suggests critical function of TGF-beta/BMP signaling in regulating epithelial-mesenchymal interaction during tooth morphogenesis. February 2003 By Xu X, Jeong L, Han J, Ito Y, Bringas P Jr, Chai Y. International Journal of Developmental Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Members of the transforming growth factor-beta family (e.g. TGF-beta, BMP and activin) ar ...

Differential expression patterns of the dentin matrix proteins during mineralized tissue formation. June 2004 By Hao J, Zou B, Narayanan K, George A. Bone You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Sequential and reciprocal interactions between the oral ectoderm and neural crest-derived mesenchyme are responsible for tooth development. During dentin formation, there are three components that are necessary for ...

Epithelial Bmpr1a regulates differentiation and proliferation in postnatal hair follicles and is essential for tooth development April 2004 By Thomas Andl, Kyung Ahn, Alladin Kairo, Emily Y. Chu, Lara Wine-Lee, Seshamma T. Reddy, Nirvana J. Croft, Judith A. Cebra-Thomas, Daniel Metzger, Pierre Chambon, Karen M. Lyons, Yuji Mishina, John T. Seykora, E. Bryan Crenshaw, III and Sarah E. Millar Development Please visit the web site to view the article in its entirety. Summary Bone morphogenet ...

EVOLUTION/DEVELOPMENT: ON THE NEURAL CREST February 2005 ScienceWeek The term "neural crest" refers to a band of embryonic ectoderm tissue on both sides of the developing neural tube. It gives rise to dorsal root ganglia, chromaffin cells, Schwann cells, and other specialized cell types. Neural crest cells often attain their final positions after lengthy migration. The neural crest itself has been believed to distinguish vertebrates from protochordates and invertebrates. The following points ...

Farewell, Tooth Fairy 10/4/04 The Osgood File (CBS Radio Network) ACFnewsource Research is being done on stem cells from tooth pulp In 2000, Dr. Songtao Shi and Dr. Stan Gronthos of the National Institute of Health (NIH) reported a surprising discovery: they found stem cells inside adult teeth. When his six-year-old daughter Julia lost a tooth, Dr. Shi inside and had a flash of inspiration—maybe baby teeth contain stem cells too? The next time his daughter lost a tooth, Shi was ready. He ru ...

Genes and tooth development: reviewing the structure and function of some key players. October/December 2003 By R. M. Scarel-Caminaga 1, S. Pasetto 2, E. Ribeiro da Silva 2, and R. C. R. Peres 2 1 University of the Sacred Heart, Bauru, São Paulo, Brazil 2 Department of Morphology, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil Braz J Oral Sci Please visit the web site to view the article in its entirety. Abstract Similar to many other embryonic orga ...

Gonadotropin releasing hormone-1 expression in incisors of mice. August 2004 By Tiong JD, Pakiam JG, Wray S. Endocrinology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract GnRH-1 is a decapeptide hormone that regulates gonadal maturation and fertility. In brain, GnRH-1 is secreted by neurons residing mainly in the preoptic/hypothalamic area. These neurons arise from cells in the nasal placode during embryonic develo ...

Grow Your Own Teeth May 4, 2004 ScienceDaily.com People who have lost or damaged teeth could soon be growing their own, thanks to a major scientific breakthrough by a start-up, Odontis Ltd, formed by King’s College, London. An investment of £400,000 from NESTA (the National Endowment for Science, Technology and the Arts) – the organisation that nurtures UK creativity and innovation and the Wellcome Trust biomedial research charity, will enable the company to move onto the next stage of develo ...

Grow-your-own to replace false teeth May 3, 2004 By Ian Sample, science correspondent The Guardian The British institution of dentures sitting in a glass of water beside the bed could be rendered obsolete by scientists who are confident that people will soon be able to replace lost teeth by growing new ones. Instead of false teeth, a small ball of cells capable of growing into a new tooth will be implanted where the missing one used to be. The procedure needs only a local anaesthetic a ...

Human Periodontal Ligament Stem Cells Isolated for the First Time July 8, 2004 National Institute of Dental and Craniofacial Research Human Periodontal Ligament Stem Cells Isolated for the First Time Over the past decade, researchers have begun sowing the scientific seeds of “regenerative dentistry,” a bold attempt to bioengineer teeth and other parts of the mouth that are frequently damaged by disease. To date, laboratories have reported early success producing tooth enamel, generating den ...

Hypothesis - Continuous tooth replacement: the possible involvement of epithelial stem cells May 24, 2004 By Ann Huysseune Irma Thesleff BioEssays You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Epithelial stem cells have been identified in integumental structures such as hairs and continuously growing teeth of various rodents, and in the gut. Here we propose the involvement of epithelial stem cells in the cont ...

Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase. August 2004 By Kamata N, Fujimoto R, Tomonari M, Taki M, Nagayama M, Yasumoto S. J Oral Pathol Med You can view the abstract online. A subscription is required to view the full text or it can be purchased online. BACKGROUND: Human telomerase reverse transcriptase (hTERT) is catalytic subunit of human telomerase. METHODS: We studied the immortaliza ...

Immunohistochemical evidence for proteolipid protein and nestin expression in the late bell stage of developing rodent teeth. February 2005 By Struys T, Krage T, Vandenabeele F, Raab WH, Lambrichts I. Archives of Oral Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract In this study, the expression of proteolipid protein (PLP) and nestin is studied in the late bell stage of developing rodent teeth in neonatal r ...

Immunolocalization of Alk8 during replacement tooth development in zebrafish. 2004 By Michael A. Perrino, Pamela C. Yelick Cells Tissues Organs You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The novel type I transforming growth factor- (TGF-) family member receptor Alk8 was previously identified in a degenerate RT-PCR screen for zebrafish type I and II TGF- family member receptors. Functional analyses revealed tha ...

Immunolocalization of bone extracellular matrix proteins (type I collagen, osteonectin and bone sialoprotein) in human dental pulp and cultured pulp cells. June 2003 By Garcia JM, Martins MD, Jaeger RG, Marques MM. Int Endod J. You can view the abstract online. A subscription is required to view the full text or it can be purchased online. AIM: To simultaneously analyse the expression of type I collagen, osteonectin and bone sialoprotein (BSP) in human dental pulp of different ages. METH ...

In baby teeth, hope for healing April 21, 2003 By SUSANNE QUICK JS Online In the future, a quarter may not be the tooth fairy's preferred under-the-pillow payment. Instead, if initial findings by a group of cell biologists at the National Institutes of Health and the Institute of Medical and Veterinary Science in Australia pan out, young children will be finding small, airtight vials filled with preserving fluid nestled under their pillows. That's because researchers have discovered that b ...

In Vitro Evaluation of the Mitogenic Effect of Platelet-Derived Growth Factor-BB on Human Periodontal Ligament Cells Cultured with Various Bone Allografts April 2003 By C.E. Papadopoulos, X.E. Dereka, E.N. Vavouraki, and I.A. Vrotsos Journal of Periodontology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Background: Several studies have documented the role of growth factors in periodontal regeneration. It has be ...

LEF1 is a critical epithelial survival factor during tooth morphogenesis. February 2005 By Sasaki T, Ito Y, Xu X, Han J, Bringas P Jr, Maeda T, Slavkin HC, Grosschedl R, Chai Y. Developmental Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. LEF1 is a cell-type-specific transcription factor and mediates Wnt signaling pathway by association with its co-activator beta-catenin. Wnt signaling is known to be critical for th ...

Lineage of non-cranial neural crest cell in the dental mesenchyme: using a lacZ reporter gene during early tooth development. 2003 By Cho SW, Hwang HJ, Kim JY, Song WC, Song SJ, Yamamoto H, Jung HS. Journal of Electron Microscopy You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The tooth is one of the ectodermal organs controlled by reciprocal interactions between the epithelium and the mesenchyme. Mesenchymal cells ...

Macrophage inflammatory protein-3alpha and beta-defensin-2 stimulate dentin sialophosphoprotein gene expression in human pulp cells. July 2003 By Shiba H, Mouri Y, Komatsuzawa H, Ouhara K, Takeda K, Sugai M, Kinane DF, Kurihara H. Biochemical and Biophysical Research Communications You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Macrophage inflammatory protein (MIP)-3alpha and beta-defensin (BD)-2 have antimicrobi ...

Mechanisms of ectodermal organogenesis. October 15, 2003 By Pispa J, Thesleff I. Developmental Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract All ectodermal organs, e.g. hair, teeth, and many exocrine glands, originate from two adjacent tissue layers: the epithelium and the mesenchyme. Similar sequential and reciprocal interactions between the epithelium and mesenchyme regulate the early steps of developme ...

Modulation of activin/bone morphogenetic protein signaling by follistatin is required for the morphogenesis of mouse molar teeth June 2004 By Wang XP, Suomalainen M, Jorgez CJ, Matzuk MM, Wankell M, Werner S, Thesleff I. Developmental Dynamics You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Teeth form as ectodermal appendages, and their morphogenesis is regulated by conserved signaling pathways. The shape of the t ...

Naturally Grown Teeth, An Alternative to Dental Implants May 3rd, 2004 AZoM™ People who have lost or damaged teeth could soon be growing their own, thanks to a major scientific breakthrough by a start-up, Odontis Ltd, formed by King’s College, London. An investment of £400,000 from NESTA (the National Endowment for Science, Technology and the Arts) – the organisation that nurtures UK creativity and innovation and the Wellcome Trust biomedial research charity, will enable the company to move o ...

Neural crest contribution to mammalian tooth formation 15 Jul 2004 By Isabelle Miletich and Paul T. Sharpe Birth Defects Research You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The cranial neural crest cells, which are specialized cells of neural origin, are central to the process of mammalian tooth development. They are the only source of mesenchyme able to sustain tooth development, and give rise not only to ...

New Insight into Progenitor/Stem Cells in Dental Pulp Using Col1a1-GFP Transgenes 2004 By Mina Mina, Alen Braut Cell Tissues Organs You can view the abstract online. A subscription is required to view the full text. Abstract In recent years there has been increasing progress in identifying stem cells from adult tissues and their potential application in tissue engineering. These advances provide a promising future for tooth replacement/regeneration. Essential for this approach is the iden ...

New perspectives on tooth development and the dental stem cell niche. March 2004 By Harada H, Ohshima H. Archives of Histology and Cytology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Adult stem cells have the capacity to self-renew and differentiate along multiple lineages in addition to contributing to ongoing tissue maintenance and regeneration after injury. They reside in specific locations called stem cell ...

New teeth 'could soon be grown' May 3, 2004 BBC News Technology to grow replacement teeth could mean the end of dentures. Scientists at King's College London have been awarded £500,000 to help them develop human teeth from stem cells. The company Odontis, set up by the college, hopes to develop its research for tests on humans within two years after successful research on mice. Stem cells, the so-called master cells, would be programmed to develop into teeth and then transplanted into t ...

Normal and abnormal dental development February 5, 2003 By Isabelle Miletich and Paul T. Sharpe, Department of Craniofacial Development, GKT Dental Institute, King's College London, Guy's Hospital, London Bridge, London SE1 9RT, UK Human Molecular Genetics Please visit the web site to view the article in its entirety. Abstract Teeth are vertebrate organs that arise from complex and progressive interactions between an ectoderm, the oral epithelium and an underlying mesenchyme. During thei ...

Odontoblasts induced from mesenchymal cells of murine dental papillae in three-dimensional cell culture August 2004 By oshi Kikuchi , Keiko Suzuki, Nobuhiro Sakai and Shoji Yamada, Department of Pharmacology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, 142-8555 Tokyo, Japan Cell and Tissue Research You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract In an organ culture system under a thre ...

PDGFR-alpha signaling is critical for tooth cusp and palate morphogenesis. January 2005 By Xu X, Bringas P Jr, Soriano P, Chai Y. Developmental Dynamics You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Platelet-derived growth factor receptor alpha (PDGFR-alpha) and PDGF ligands are key regulators for embryonic development. Although Pdgfralpha is spatially expressed in the cranial neural crest (CNC)-derived odontogen ...

Potent Stem Cells Found in Baby Teeth April 23, 2003 By Laura Wright Scientific American, Inc. Your seven-year-old's baby tooth may be worth a lot more than the quarter the tooth fairy left under the pillow. Scientists have discovered that the pulp inside deciduous teeth is a treasure trove of fast-growing stem cells. Naturally-shed choppers could thus provide an easily accessible new source of these sought-after cells for clinical studies of stem-cell transplantation and tissue engineerin ...

Progenitor cells from dental follicle are able to form cementum matrix in vivo. 2004 By Handa K, Saito M, Tsunoda A, Yamauchi M, Hattori S, Sato S, Toyoda M, Teranaka T, Narayanan AS. Connective Tissue Research You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract: To address the molecular mechanisms of cementogenesis, we have isolated dental follicle cells and examined them to see if they contain cementoblast progenit ...

Prospects for tooth regeneration in the 21st century: a perspective. April 1, 2003 By Chai Y, Slavkin HC. Microscopy Research and Technique You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The prospects for tooth regeneration in the 21st century are compelling. Using the foundations of experimental embryology, developmental and molecular biology, the principles of biomimetics (the mimicking of biological processes) ...

PRP to Enhance Pre-Prosthetic Bone Grafts, Dental Implants and Periodontics PRP Central Although bone augmentation for supporting osseointegrated dental implants has been a routine technique in dentistry for more than a decade, research continues toward improving our ability to manage bone grafts. Most recently, researchers have focused on the plausability of applying polypeptide growth and differentiation factors (GDFs) to enhance bone regeneration. GDFs are a class of biologic mediators th ...

Regulatory mechanisms of periodontal regeneration February 2003 By Masaki Shimono, Tatsuya Ishikawa, Hiroki Ishikawa, Hirokazu Matsuzaki , Sadamitsu Hashimoto, Takashi Muramatsu, Kaori Shima, Ken-Ichi Matsuzaka, Takashi Inoue Microscopy Research and Technique You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The periodontal ligament, located between the cementum and the alveolar bone, has a width ranging from 0.15 ...

Researchers grow teeth in the lab Oct. 15, 2003 Associated Press MSNBC BOSTON - A team of scientists grew accurate versions of natural teeth in a laboratory, raising the possibility of an eventual replacement for manmade implants such as dentures, bridges and crowns. The scientists, based at Forsyth Institute, were reportedly the first to grow such a complex tooth structure from a collection of individual cells. “This is very exciting, and I don’t think it’s expected,” said R. Bruce Dono ...

Role of Hertwig's epithelial root sheath cells in tooth root development October 2003 By Margarita Zeichner-David , Keiji Oishi, Zhengyan Su, Vassili Zakartchenko, Li-Sha Chen, Higinio Arzate, Pablo Bringas Jr. Developmental Dynamics You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract During tooth development, after the completion of crown formation, the apical mesenchyme forms the developing periodontium while the i ...

Role of Islet1 in the patterning of murine dentition 2003 By Thimios A. Mitsiadis, Irene Angeli, Chela James, Urban Lendahl and Paul T. Sharpe Development Please visit the web site to view the article in its entirety. Summary It is believed that mouse dentition is determined by a prepatterning of the oral epithelium into molar (proximal) and incisor (distal) regions. The LIM homeodomain protein Islet1 (ISL1) is involved in the regulation of differentiation of many cell types and organs. D ...

Root or crown: a developmental choice orchestrated by the differential regulation of the epithelial stem cell niche in the tooth of two rodent species 2003 By Mark Tummers and Irma Thesleff Development Please visit the web site to view the article in its entirety. Summary The rodent incisor grows continuously throughout its lifetime. The epithelial stem cell niche is located at the apical end of the tooth and its progeny gives rise to the ameloblasts that form the hard enamel. Previously ...

Scientists Discover Unique Source Of Postnatal Stem Cells in 'Baby' Teeth April 22, 2003 ScienceDaily.com Scientists report for the first time that "baby" teeth, the temporary teeth that children begin losing around their sixth birthday, contain a rich supply of stem cells in their dental pulp. The researchers say this unexpected discovery could have important implications because the stem cells remain alive inside the tooth for a short time after it falls out of a child's mouth, suggesting t ...

Scientists plan teeth that regrow May 4, 2004 CNN.com LONDON, England -- False teeth could become a thing of the past thanks to stem cell technology, scientists at London University say. Successful tests on mice show the technology may let people grow their own replacement teeth. The London team at Kings College have been awarded £500,000 ($887,500) to further their research and have set up a private company, Odontis, to develop their plans. The scientists say the technology will allow th ...

Scientists Report Important New Data in Adult Stem Cell Debate March 27, 2003 National Institute of Dental and Craniofacial Research Developmental biologists have long maintained that adult stem cells cannot be reprogrammed. Once a stem cell commits to a specific tissue, such as the brain, it can’t turn back its biological clock and become blood, bone, or any other type of adult stem cell. But, about four years ago, this fundamental idea received a serious jolt when scientists reported indu ...

SEM observations of the attachment of human periodontal ligament fibroblasts to non-demineralized dentin surface in vitro. March 2004 By Al-Nazhan S. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics You can view the abstract online. A subscription is required to view the full text or it can be purchased online. PURPOSE: The purpose of this investigation was to study in-vitro the attachment behavior of human periodontal ligament (HPDL) fibroblasts to nondemineralize ...

SHED: Stem cells from human exfoliated deciduous teeth May 13, 2003 By Masako Miura*, Stan Gronthos 1, Mingrui Zhao 3, Bai Lu 1, Larry W. Fisher*, Pamela Gehron Robey*, and Songtao Shi*,§ * Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892; 1 Mesenchymal Stem Cell Group, Division of Haematology, Institute of Medical and Veterinary Science, Frome Road, Adelaide 5000, South Australia, Austral ...

Stem cell responses in tooth regeneration. June 2004 By Murray PE, Garcia-Godoy F. Stem Cells Dev You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Scientific advances in the creation of restorative biomaterials, in vitro cell culture technology, tissue grafting, tissue engineering, molecular biology, and the human genome project provide the basis for the introduction of new technologies into dentistry. This review i ...

Stem Cells and Tissue Engineering: Prospects for Regenerating Tissues in Dental Practice 2003 By Irma Thesleff, Mark Tummers International Journal of the Kuwait University Health Sciences Centre You can view the abstract online. A subscription is required to view the full text. Abstract In general, human tissues have a very limited potential to regenerate. However, recent progress in stem cell research and in tissue engineering promises novel prospects for tissue regeneration in dental pr ...

Stem cells and tissue engineering: prospects for regenerating tissues in dental practice. April 2003 By Thesleff I, Tummers M. Medical Principles and Practice You can view the abstract online. A subscription is required to view the full text. Abstract In general, human tissues have a very limited potential to regenerate. However, recent progress in stem cell research and in tissue engineering promises novel prospects for tissue regeneration in dental practice in the future. Stem cells hav ...

Stem cells in tooth pulp could be used in research May 3, 2003 By Deborah Josefson British Medical Journal Researchers from the United States and Australia have found that deciduous teeth have robust stem cells in their dental pulp. The finding is important, because such teeth may serve as an easily obtainable alternative to embryonic stem cells, the use of which has proved controversial. Moreover, like umbilical cord blood, they may serve as a ready source of autologous and bankable stem ...

Stimulation of reparative dentin formation by ex vivo gene therapy using dental pulp stem cells electrotransfected with growth/differentiation factor 11 (Gdf11). November 2004 By Nakashima M, Iohara K, Ishikawa M, Ito M, Tomokiyo A, Tanaka T, Akamine A. Human Gene Therapy You can view the abstract online. A subscription is required to view the full text. Dental pulp progenitor/stem cells have the capacity to differentiate into odontoblasts and they provide a potential for dentin repair and ...

Taken from Teeth, Stem Cells Regenerate Tissue August 13, 2004 Genome News Network Researchers have isolated stem cells from the ligament that holds human teeth in place and shown that, in laboratory mice, these cells are able to grow new ligament cells. In particular, they were able to grow a particular type of cell, known as Sharpey's fiber, needed for a tooth to attach to its socket. The researchers, led by Songtao Shi of the National Institute of Dental and Craniofacial Research in Beth ...

TGF-ß SIGNALING AND ITS FUNCTIONAL SIGNIFICANCE IN REGULATING THE FATE OF CRANIAL NEURAL CREST CELLS 2003 By Y. Chai, Y. Ito, J. Han Critical Reviews in Oral Biology & Medicine Please visit the web site to view the article in its entirety. Abstract Members of the transforming growth factor-ß (TGF-ß) superfamily regulate cell proliferation, differentiation, and apoptosis, and control the development and maintenance of most tissues. TGF-ß signal is transmitted through the phosphorylation ...

The Developing Mouse Dentition 2003 By RENATA PETERKOVÁ, MIROSLAV PETERKA and HERVÉ LESOT Annals of the New York Academy of Science You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Developing limb or differentiating neural and blood cells are traditional models used to study programmed cell death in mammals. The developing mouse dentition can also be an attractive model for studying apoptosis regulation. Apoptosis ...

Timing of odontogenic neural crest cell migration and tooth-forming capability in mice February 2003 By Zhang Y, Wang S, Song Y, Han J, Chai Y, Chen Y. Developmental Dynamics You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The mammalian tooth develops through sequential and reciprocal interactions between cranial neural crest (CNC)- derived ectomesenchymal cells and the stomadial epithelium. Classic tissue recombi ...

Tissue engineering regenerates tooth crowns in animal model June 28, 2004 By Mark Berthold American Dental Association Boston — Researchers from the Forsyth Institute have regenerated tooth crowns in rats, according to a new study. "Bioengineered teeth from cultured rat tooth bud cells," which appears in July's Journal of Dental Research, reports maintaining individual tooth-forming cells in culture for six days before implanting them. According to the researchers — from Forsyth, Massachu ...

To the root of the stem cell problem - The evolutionary importance of the epithelial stem cell niche during tooth development September 2004 By Mark Tummers, Developmental Biology Programme Institute of Biotechnology. University of Helsinki and Viikki Graduate School in Biosciences, University of Helsinki - Academic Dissertation Please visit the web site to view the article in its entirety. SUMMARY A tooth is an ectodermal organ and its development relies on epithelial-mesenchymal interacti ...

Tooth and jaw: molecular mechanisms of patterning in the first branchial arch. January 2003 By Cobourne MT, Sharpe PT. Archives of Oral Biology You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract The mammalian jaw apparatus is ultimately derived from the first branchial arch derivatives, the maxillary and mandibular processes, and composed of a highly specialised group of structures. Principle amongst these are the sk ...

Tooth growing experiments bring smiles June 24, 2004 By James Randerson New Scientist Remarkable progress has been made towards growing replacement teeth from stem cells, according to a paper published this week. But major obstacles remain before this could be attempted in people. Paul Sharpe's team at King's College London is one of several worldwide attempting to grow replacement teeth (New Scientist print edition, 10 August 2002, p 32). There was a flurry of publicity about Sharpe's wor ...

Traf6 is essential for murine tooth cusp morphogenesis November 2003 By Ohazama A, Courtney JM, Tucker AS, Naito A, Tanaka S, Inoue J, Sharpe PT. Developmental Dynamics You can view the abstract online. A subscription is required to view the full text or it can be purchased online. Abstract Ectodermal appendages such as skin, hair, teeth, and sweat glands are affected in patients with hypohidrotic (anhydrotic) ectodermal dysplasia (HED). It has been established that mutations in the tumo ...

U.S.- Brazil Team Bioengineers Tooth Crowns In Second Mammal Species June 25, 2004 ScienceDaily.com Boston, MA -- Researchers at The Forsyth Institute and Massachusetts General Hospital in Boston and Universidade Federal de Sao Paulo (UNIFESP) in Brazil have successfully used tissue-engineering techniques to regenerate rat tooth crowns. The advance follows Forsyth’s widely-publicized regeneration of pig tooth crowns in 2002 and adds evidence that it may, one day, be possible to grow new huma ...